SYNTHESIS OF BRANCHED OLIGOSACCHARIDES FROM STARCH BY 2 AMYLASES CLONED FROM BACILLUS-LICHENIFORMIS

被引：15

作者：

KIM, IC

YOO, SH

LEE, SJ

OH, BH

KIM, JW

PARK, KH

机构：

[1] SEOUL NATL UNIV, COLL AGR & LIFE SCI, NEW BIOMAT AGR RES CTR, SUWON 441774, SOUTH KOREA

[2] SEOUL NATL UNIV, COLL AGR & LIFE SCI, DEPT FOOD SCI & TECHNOL, SUWON 441774, SOUTH KOREA

[3] SMITHKLINE BEECHAM PHARMACEUT, DEPT MACROMOLEC SCI, KING OF PRUSSIA, PA 19406 USA

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1994年 / 58卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

D O I：

10.1271/bbb.58.416

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An E. coli cell extract containing thermostable a-amylase and maltogenic amylase expressed from the clone pTMA322 was used to produce an anomalously linked oligosaccharides (Ale) mixture from starch. The liquefaction and saccharification of starch were done by one-step procedure using the above cell extract. The resulted Alo mixture contained over 40% oligosaccharides having DP 3 or more including branched forms.

引用

页码：416 / 418

页数：3

共 5 条

[1] KIM IC, 1992, J BIOL CHEM, V267, P22108
[2] KIM IC, 1991, THESIS SEOUL NATIONA
[3] TAKAKU H, 1988, HDB AMYLASE RELATED, P215
[4] Yu, 1992, J APPL MICROBIOL BIO, V20, P237
[5] COMPLETE NUCLEOTIDE-SEQUENCE OF A GENE CODING FOR HEAT-STABLE AND PH-STABLE ALPHA-AMYLASE OF BACILLUS-LICHENIFORMIS - COMPARISON OF THE AMINO-ACID SEQUENCES OF 3 BACTERIAL LIQUEFYING ALPHA-AMYLASES DEDUCED FROM THE DNA-SEQUENCES
YUUKI, T
NOMURA, T
TEZUKA, H
TSUBOI, A
YAMAGATA, H
TSUKAGOSHI, N
UDAKA, S
[J]. JOURNAL OF BIOCHEMISTRY, 1985, 98 (05) : 1147 - 1156

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