DETECTION OF BINDING OF DENATURED SALIVARY ALPHA-AMYLASE TO STREPTOCOCCUS-SANGUIS

被引：4

作者：

BERGMANN, JE

GULZOW, HJ

机构：

[1] University of Hamburg, Dental School, Dept. of Conservative and Preventive Dentistry, 20246 Hamburg

来源：

ARCHIVES OF ORAL BIOLOGY | 1995年 / 40卷 / 10期

关键词：

STREPTOCOCCUS SANGUIS; SALIVARY ALPHA-AMYLASE; BINDING SITE; OVERLAY TECHNIQUE;

D O I：

10.1016/0003-9969(95)00070-6

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Native alpha-amylase, either solubilized, or immobilized and tested with an overlay immunotechnique, was bound in a species-specific manner to Streptococcus mitis and to one of the Streptococcus gordonii strains. However, only insignificant amounts of alpha-amylase were bound to Streptococcus sanguis and all other strains tested. When alpha-amylase was denatured before immobilization, Streptococcus sanguis bound strongly to the protein while binding of other strains was insignificant.

引用

页码：973 / 974

页数：2

共 7 条

[1] Bergmann J E, 1990, Dtsch Zahnarztl Z, V45, P733
[2] BERGMANN JE, 1993, DTSCH ZAHNARZTL Z, V48, P634
[3] THE BINDING OF HUMAN SALIVARY ALPHA-AMYLASE BY ORAL STRAINS OF STREPTOCOCCAL BACTERIA
DOUGLAS, CWI
[J]. ARCHIVES OF ORAL BIOLOGY, 1983, 28 (07) : 567 - 573
[4] DOUGLAS CWI, 1990, FEMS MICROBIOL LETT, V54, P193
[5] ABILITY TO BIND SALIVARY ALPHA-AMYLASE DISCRIMINATES CERTAIN VIRIDANS GROUP STREPTOCOCCAL SPECIES
KILIAN, M
NYVAD, B
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (11) : 2576 - 2577
[6] STREPTOCOCCUS-SANGUIS SURFACE-ANTIGENS AND THEIR INTERACTIONS WITH SALIVA
LAMONT, RJ
ROSAN, B
MURPHY, GM
BAKER, CT
[J]. INFECTION AND IMMUNITY, 1988, 56 (01) : 64 - 70
[7] ADHERENCE OF ORAL STREPTOCOCCI TO SALIVARY GLYCOPROTEINS
MURRAY, PA
PRAKOBPHOL, A
LEE, T
HOOVER, CI
FISHER, SJ
[J]. INFECTION AND IMMUNITY, 1992, 60 (01) : 31 - 38

← 1 →