DEMONSTRATION OF POSITIONALLY DISORDERED WATER WITHIN A PROTEIN HYDROPHOBIC CAVITY BY NMR

被引：243

作者：

ERNST, JA ^{[1
]}

CLUBB, RT ^{[1
]}

ZHOU, HX ^{[1
]}

GRONENBORN, AM ^{[1
]}

CLORE, GM ^{[1
]}

机构：

[1] NIDDKD, CHEM PHYS LAB, BETHESDA, MD 20892 USA

来源：

SCIENCE | 1995年 / 267卷 / 5205期

关键词：

D O I：

10.1126/science.7892604

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The presence and location of water of hydration (thai is, bound water) in the solution structure of human interleukin-1 beta (hIL-1 beta) was investigated with water-selective two-dimensional heteronuclear magnetic resonance spectroscopy. It is shown here that in addition to water al the surface of the protein and ordered internal water molecules involved in bridging hydrogen bonds, positionally disordered water is present within a large, naturally occurring hydrophobic cavity located at the center oi the molecule. These water molecules of hydration have residency times in the range of 1 to 2 nanoseconds to 100 to 200 microseconds and can be readily detected by nuclear magnetic resonance (NMR). Thus, large hydrophobic cavities in proteins may not be truly empty, as analysis oi crystal structures appears to show, but may contain mobile water molecules that are crystallographically invisible but detectable by NMR.

引用

页码：1813 / 1817

页数：5

共 49 条

[1] SOLVATION THERMODYNAMICS OF NONIONIC SOLUTES [J].