NITROXIDES IN THE SOLUTION OF SOME PROBLEMS OF CHEMICAL BIOPHYSICS

The efficiency of.the nitroxide radicals (NR) unravelle biophysical problem increases markedly by use of combine approach including: 1. Measuring of relaxation characteristics of the ESR signals of NR; 2. Study of NR effect on exited triplet stated of chromophores; 3. High resolution (2 mm band) ESR technique; 4. Use of methods fluorescent, triplet, mossbauer and radicals pairs labeling. Results of study of molecular dynamics in wide range of correlation time values, protein molecules conductivity for spin exchange exxects, long-distance electron transfer, structures of a number complex enzymatic systems (nitrogenase, reaction center of photosynthetic bacteria and cytochrome P-450, etc.) have been reported. Some new conceptions which have been introduced in connection with these results, namely, multi-electron mechanisms of redox processes, chemical mechanism of energetic coupling, cascade scheme of photoseparation at photosynthesis, role molecular dynamics, exchange phenomena and complexation in electron transfer have been discussed. © 1990 De Gruyter