PROPERTIES OF OXIDIZED AND REDUCED SPINACH (SPINACIA-OLERACEA) CHLOROPLAST FRUCTOSE-1,6-BISPHOSPHATASE ACTIVATED BY VARIOUS AGENTS

被引:9
作者
CHARDOT, T [1 ]
MEUNIER, JC [1 ]
机构
[1] INRA,CTR BIOTECHNOL AGROIND,F-78850 THIVERVAL GRIGNON,FRANCE
关键词
D O I
10.1042/bj2780787
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fructose-1,6-bisphosphatase (FBPase) can be reduced and activated by either dithiothreitol or reduced thioredoxin. This activation is pH-dependent. An amino acid group with a pK value of 5.55 is involved in this process. Both enzyme forms can also be stimulated by agents such a fructose 1,6-bisphosphate, Mg2+, Ca2+ and Ca2+/fructose 1,6-bisphosphate. FBPase reduced by dithiothreitol is more strongly activated than the enzyme reduced by thioredoxin. The specificity constant (k(cat). / k(m)) is enhanced over 2.5-25-fold and 1.5-2-fold (depending on the agent used) for FBPase reduced by dithiothreitol and thioredoxin respectively. In both cases, no new kinetic properties appeared. The pH-activity profile of the stimulated enzyme is slightly shifted towards the acidic side with respect to the reduced enzyme. A lag phase is observed in the progress curve of both enzymic forms, treated or untreated. Each agent used to stimulate must induce a new conformation of the enzyme, more active than the initial one, characterized by a specificity constant and a relaxation time. This lag phase tends to disappear when the assay temperature is increased. Temperature has the same effect on the activity of oxidized, reduced and stimulated FBPase, but different effects on the stability of the different forms.
引用
收藏
页码:787 / 791
页数:5
相关论文
共 19 条
[1]   AFFINITY-CHROMATOGRAPHY, ON FRUCTOSE-BISPHOSPHATASE SEPHAROSE, OF 2 CHLOROPLASTIC THIOREDOXINS-F - PURIFICATION AND COMPARATIVE MOLECULAR-PROPERTIES [J].
BUC, J ;
RIVIERE, M ;
GONTERO, B ;
SAUVE, P ;
MEUNIER, JC ;
RICARD, J .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1984, 140 (01) :199-202
[2]   EFFECTS OF PH AND FRUCTOSE 2,6-BISPHOSPHATE ON OXIDIZED AND REDUCED SPINACH CHLOROPLASTIC FRUCTOSE-1,6-BISPHOSPHATASE [J].
CADET, F ;
MEUNIER, JC ;
FERTE, N .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1987, 162 (02) :393-398
[3]   FRUCTOSE-1,6-BISPHOSPHATE AND CALCIUM ACTIVATE OXIDIZED SPINACH (SPINACIA OLERACEA) CHLOROPLAST FRUCTOSE-1,6-BISPHOSPHATASE [J].
CHARDOT, T ;
MEUNIER, JC .
PLANT SCIENCE, 1990, 70 (01) :1-9
[4]   ACTION OF CALCIUM-IONS ON SPINACH (SPINACIA-OLERACEA) CHLOROPLAST FRUCTOSE BISPHOSPHATASE AND OTHER ENZYMES OF THE CALVIN CYCLE [J].
CHARLES, SA ;
HALLIWELL, B .
BIOCHEMICAL JOURNAL, 1980, 188 (03) :775-779
[5]   EVIDENCE FOR FUNCTION OF THE FERREDOXIN THIOREDOXIN SYSTEM IN THE REDUCTIVE ACTIVATION OF TARGET ENZYMES OF ISOLATED INTACT CHLOROPLASTS [J].
CRAWFORD, NA ;
DROUX, M ;
KOSOWER, NS ;
BUCHANAN, BB .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1989, 271 (01) :223-239
[6]  
FRIEDEN C, 1970, J BIOL CHEM, V245, P5788
[7]   ON THE ACTIVATION OF 2 CHLOROPLASTIC PHOSPHATASES BY FRUCTOSE BISPHOSPHATE, SEDOHEPTULOSE BISPHOSPHATE AND MAGNESIUM [J].
GONTERO, B ;
MEUNIER, JC ;
RICARD, J .
PLANT SCIENCE LETTERS, 1984, 36 (03) :195-199
[8]   ANALYTICAL ULTRACENTRIFUGATION STUDIES ON CHLOROPLASTIC FRUCTOSE BISPHOSPHATASE [J].
GONTERO, B ;
MEUNIER, JC ;
SAUVE, P ;
RICARD, J .
PLANT SCIENCE, 1985, 38 (01) :17-22
[9]  
HERTIG CM, 1983, J BIOL CHEM, V258, P984
[10]   THE ROLE OF PH AND MAGNESIUM CONCENTRATION IN THE LIGHT ACTIVATION OF CHLOROPLASTIC FRUCTOSE BISPHOSPHATASE [J].
MINOT, R ;
MEUNIER, JC ;
BUC, J ;
RICARD, J .
FEBS LETTERS, 1982, 142 (01) :118-120