ISOLATION, CHARACTERIZATION AND MAPPING OF PYRIMIDINE AUXOTROPHS OF PHYCOMYCES-BLAKESLEEANUS

被引：7

作者：

CAMPUZANO, V ^{[1
]}

DELVALLE, P ^{[1
]}

DEVICENTE, JI ^{[1
]}

ESLAVA, AP ^{[1
]}

ALVAREZ, MI ^{[1
]}

机构：

[1] UNIV SALAMANCA,FAC BIOL,DEPT MICROBIOL & GENET,AREA GENET,E-37008 SALAMANCA,SPAIN

来源：

CURRENT GENETICS | 1993年 / 24卷 / 06期

关键词：

PHYCOMYCES; PYRF; PYRG; COMPLEMENTATION; RECOMBINATION;

D O I：

10.1007/BF00351715

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A total of seven pyrimidine auxotrophs of Phycomyces were isolated from among 5-fluoroorotate acid (5-FOA)-resistant mutants. They were classified by complementation into two groups. A representative mutant strain belonging to one group was deficient in orotate phosphoribosyl transferase (OPRTase; EC 2.4.2.10) activity; the mutant strain belonging to the second group was deficient in orotidine-5'-monophosphate decarboxylase (OMPdecase; EC 4.1.1.23). These mutants are defective in the genes pyrF and pyrG respectively. The results from random spore analysis, tetrad analysis, and gene-centromere distances showed that these two markers are located in linkage group VI, with pyrG being a proximal marker and pyrF a distal one.

引用

页码：515 / 519

页数：5

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