ENHANCED RECOMBINATION BETWEEN LAMBDA-PLAC5 AND MINI-F-LAC - THE TRA-REGULON IS REQUIRED FOR RECOMBINATION ENHANCEMENT

被引：22

作者：

SEIFERT, HS ^{[1
]}

PORTER, RD ^{[1
]}

机构：

[1] PENN STATE UNIV, DEPT BIOCHEM MICROBIOL MOLEC & CELL BIOL, MICROBIOL PROGRAM, UNIVERSITY PK, PA 16802 USA

来源：

MOLECULAR AND GENERAL GENETICS | 1984年 / 193卷 / 02期

关键词：

D O I：

10.1007/BF00330679

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

F42lac recombination with .lambda.plac is normally 20- to 50-fold higher than recombination between .lambda.plac and a chromosomal lac gene. Transductional crosses with .lambda.plac and a recombinant plasmid containing the lac operon and F replication functions show only 2- to 4-fold higher recombination than similar crosses with a chromosomal lac gene. Insertion of a BamHI fragment containing the entire tra regulon of F into the min-F-lac plasmid restores the high level of recombination seen with F42lac.

引用

页码：269 / 274

页数：6

共 28 条

[1] PEDIGREES OF SOME MUTANT STRAINS OF ESCHERICHIA-COLI K-12 [J].

BACHMANN, BJ .

BACTERIOLOGICAL REVIEWS, 1972, 36 (04) :525-557

[2] DETECTION OF TRANSCRIBABLE RECOMBINATION PRODUCTS FOLLOWING CONJUGATION IN REC+, RECB-STRAINS AND RECC-STRAINS OF ESCHERICHIA-COLI-K12 [J].

BIRGE, EA ;

LOW, KB .

JOURNAL OF MOLECULAR BIOLOGY, 1974, 83 (04) :447-457

[3]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[4] INVITRO AND INVIVO MANIPULATIONS OF BACTERIOPHAGE MU DNA - CLONING OF MU ENDS AND CONSTRUCTION OF MINI-MUS CARRYING SELECTABLE MARKERS [J].

CHACONAS, G ;

DEBRUIJN, FJ ;

CASADABAN, MJ ;

LUPSKI, JR ;

KWOH, TJ ;

HARSHEY, RM ;

DUBOW, MS ;

BUKHARI, AI .

GENE, 1981, 13 (01) :37-46

[5] SUPERCOILED CIRCULAR DNA-PROTEIN COMPLEX IN ESCHERICHIA COLI - PURIFICATION AND INDUCED CONVERSION TO AN OPEN CIRCULAR DNA FORM [J].

CLEWELL, DB ;

HELINSKI, DR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1969, 62 (04) :1159-&

[6] RECIRCULARIZATION AND AUTONOMOUS REPLICATION OF A SHEARED R-FACTOR DNA SEGMENT IN ESCHERICHIA-COLI TRANSFORMANTS - (PLASMID-TRANSFORMATION-ANTIBIOTIC RESISTANCE DNA) [J].

COHEN, SN ;

CHANG, ACY .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (05) :1293-1297

[7] PROLONGED INCUBATION IN CALCIUM-CHLORIDE IMPROVES THE COMPETENCE OF ESCHERICHIA-COLI-CELLS [J].

DAGERT, M ;

EHRLICH, SD .

GENE, 1979, 6 (01) :23-28

[8] LIGATION OF ECORI ENDONUCLEASE-GENERATED DNA FRAGMENTS INTO LINEAR AND CIRCULAR STRUCTURES [J].

DUGAICZYK, A ;

BOYER, HW ;

GOODMAN, HM .

JOURNAL OF MOLECULAR BIOLOGY, 1975, 96 (01) :171-&

[9] RAPID METHOD FOR IDENTIFICATION OF PLASMID DEOXYRIBONUCLEIC-ACID IN BACTERIA [J].

ECKHARDT, T .

PLASMID, 1978, 1 (04) :584-588

[10] BIDIRECTION REPLICATION FROM A UNIQUE ORIGIN IN A MINI-F PLASMID [J].

EICHENLAUB, R ;

FIGURSKI, D ;

HELINSKI, DR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (03) :1138-1141

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