GUANINE NUCLEOTIDE-INDUCED POSITIVE COOPERATIVITY IN MUSCARINIC-CHOLINERGIC ANTAGONIST BINDING

被引：23

作者：

BOYER, JL

MARTINEZCARCAMO, M

MONROYSANCHEZ, JA

POSADAS, C

GARCIASAINZ, JA

机构：

[1] NATL AUTONOMOUS UNIV MEXICO, INST FISIOL CELULAR, DEPT BIOENERGET, APARTADO POSTAL 70-600, MEXICO CITY 04510, DF, MEXICO

[2] INST NACL CARDIOL DR IGNACIO CHAVEZ, DEPT ENDOCRINOL, JUAN BADIANO 1, MEXICO 14080, DF, MEXICO

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1986年 / 134卷 / 01期

关键词：

D O I：

10.1016/0006-291X(86)90543-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of guanine nucleotides on the binding of [3H]Quinuclidinyl benzilate to heart muscarinic receptors was studied. It was observed that GTP and Gpp(NH)p increased antagonist binding. Scatchard transformation of the data resulted in curvilinear plots (downward concavity) consistent with positive cooperativity. Graphic analysis for cooperativity indicated that guanine nucleotides: (a) increased slightly but consistently the affinity for the antagonist (independent of receptor occupancy) and (b) induce positive cooperativity in the binding of [3H]QNB (dependent of receptor occupancy).

引用

页码：172 / 177

页数：6

共 19 条

[1] GUANINE-NUCLEOTIDES MODULATE MUSCARINIC RECEPTOR-BINDING IN THE HEART [J].

BERRIE, CP ;

BIRDSALL, NJM ;

BURGEN, ASV ;

HULME, EC .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1979, 87 (04) :1000-1005

[2]

BIRDSALL NJ, 1978, MOL PHARM, V14, P726

[3] RECIPROCAL MODULATION OF AGONIST AND ANTAGONIST BINDING TO MUSCARINIC CHOLINERGIC RECEPTOR BY GUANINE-NUCLEOTIDE [J].

BURGISSER, E ;

DELEAN, A ;

LEFKOWITZ, RJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (06) :1732-1736

[4] GTP INCREASES AIRWAY MUSCARINIC ANTAGONIST BINDING-SITES - AN EFFECT REGULATED BY MG-2+ [J].

CHENG, JB ;

TOWNLEY, RG .

EUROPEAN JOURNAL OF PHARMACOLOGY, 1983, 88 (2-3) :269-272

[5]

DELEAN A, 1979, RECEPTORS, V1, P143

[6] COOPERATIVITY IN LIGAND-BINDING - NEW GRAPHIC ANALYSIS [J].

DEMEYTS, P ;

ROTH, J .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1975, 66 (04) :1118-1126

[7]

EHLERT FJ, 1981, FED PROC, V40, P153

[8] INTERACTIONS OF AGONISTS WITH PLATELET ALPHA-2-ADRENERGIC RECEPTORS [J].

HOFFMAN, BB ;

MICHEL, T ;

BRENNEMAN, TB ;

LEFKOWITZ, RJ .

ENDOCRINOLOGY, 1982, 110 (03) :926-932

[9] REGULATION OF ANTAGONIST BINDING TO CARDIAC MUSCARINIC RECEPTORS [J].

HOSEY, MM .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1982, 107 (01) :314-321

[10] 2 POPULATIONS OF BINDING-SITES FOR MUSCARINIC ANTAGONISTS IN THE RAT-HEART [J].

HULME, EC ;

BERRIE, CP ;

BIRDSALL, NJM ;

BURGEN, ASV .

EUROPEAN JOURNAL OF PHARMACOLOGY, 1981, 73 (2-3) :137-142

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