FLOW-INJECTION AMPEROMETRIC SYSTEM FOR ENZYME-IMMUNOASSAY

被引：21

作者：

IVNITSKII, DM

SITDIKOV, RA

KUROCHKIN, VE

机构：

[1] Department of Chemistry, Medical Institute

来源：

ANALYTICA CHIMICA ACTA | 1992年 / 261卷 / 1-2期

关键词：

AMPEROMETRY; FLOW SYSTEM; IMMUNOASSAY; ENZYME-LINKED IMMUNOSORBENT ASSAY; IMMUNOGLOBULIN-E;

D O I：

10.1016/0003-2670(92)80174-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A flow-injection amperometric system was developed for monitoring the results of enzyme immunoassay. The operation of the flow-through amperometric detector is described by a limiting current equation for the working wire electrode. The possibility of the optimization of the geometry and hydrodynamic properties of amperometric detectors in flow systems was demonstrated. The flow system was tested in the determination of peroxidase and immunoglobulin E in blood serum. Peroxidase (marker enzyme) was determined in the concentration range 5 x 10(-12)-4 X 10(-10) M. The detection limit for immunoglobulin E in blood serum was 3.1 ng ml-1. The sample throughput was 400 h-1.

引用

页码：45 / 52

页数：8

共 19 条

[1] ENZYME IMMUNOSENSOR .3. AMPEROMETRIC DETERMINATION OF HUMAN CHORIONIC-GONADOTROPIN BY MEMBRANE-BOUND ANTIBODY
AIZAWA, M
MORIOKA, A
SUZUKI, S
NAGAMURA, Y
[J]. ANALYTICAL BIOCHEMISTRY, 1979, 94 (01) : 22 - 28
[2] BOITIEUX JL, 1979, CLIN CHEM, V25, P318
[3] EGGERS HM, 1982, CLIN CHEM, V28, P1848
[4] IVNITSKII DM, 1988, LAB DELO, V8, P52
[5] IVNITSKII DM, 1989, ZH ANAL KHIM, V44, P151
[6] IVNITSKII DM, 1986, ZH ANAL KHIM, V41, P55
[7] KOLEV SD, 1988, ANAL CHEM, V60, P700
[8] EFFECT OF VARIATION IN FLOW-RATE ON AMPEROMETRIC DETECTION IN FLOW-INJECTION ANALYSIS
MACKOUL, D
JOHNSON, DC
SCHICK, KG
[J]. ANALYTICAL CHEMISTRY, 1984, 56 (03) : 436 - 439
[9] ENZYME IMMUNOELECTRODE - ASSAY OF HUMAN-SERUM ALBUMIN AND INSULIN
MATTIASSON, B
NILSSON, H
[J]. FEBS LETTERS, 1977, 78 (02) : 251 - 254
[10] MELNIK S, 1987, CHEM LISTY, V81, P243

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