A SIMPLE AND RAPID METHOD FOR ISOLATION OF EOSINOPHILIC GRANULOCYTES FROM HUMAN BLOOD

被引:27
作者
CRAMER, R
DRI, P
ZABUCCHI, G
PATRIARCA, P
机构
[1] Institute of General Pathology, University of Trieste, 34127 Trieste
关键词
EOSINOPHILS; CELL SEPARATION; EOSINOPHILIA; SUPEROXIDE ANION; CELL DENSITY;
D O I
10.1002/jlb.52.3.331
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
A simple and rapid method for the purification of morphologically and functionally intact eosinophils from human blood of both normal and eosinophilic subjects is described. The method is based on a single centrifugation of total blood leukocytes suspended in Percoll with specific gravity 1.0853 g/ml, after erythrocyte removal by dextran sedimentation. The peculiarity of this isolation technique is the maintenance of strictly physiological values of pH and osmolality throughout the entire procedure. Moreover, the cells are not subjected to measures aimed at changing the physical properties of either neutrophils or eosinophils. Because of such characteristics, this isolation method could be usefully exploited for comparative studies of normal and eosinophilic normodense eosinophils and of neutrophils and eosinophils from the same noneosinophilic subject.
引用
收藏
页码:331 / 336
页数:6
相关论文
共 21 条
[1]   COMPARISON OF HUMAN EOSINOPHILS FROM NORMALS AND PATIENTS WITH EOSINOPHILIA [J].
BASS, DA ;
GROVER, WH ;
LEWIS, JC ;
SZEJDA, P ;
DECHATELET, LR ;
MCCALL, CE .
JOURNAL OF CLINICAL INVESTIGATION, 1980, 66 (06) :1265-1273
[2]  
BRATTIG NW, 1987, EUR J HAEMATOL, V39, P148
[3]  
DESIMONE C, 1982, CLIN EXP IMMUNOL, V48, P249
[4]  
DRI P, 1991, J IMMUNOL, V147, P613
[5]   ROLE OF MYELOPEROXIDASE IN RESPIRATORY BURST OF HUMAN POLYMORPHONUCLEAR LEUKOCYTES - STUDIES WITH MYELOPEROXIDASE-DEFICIENT SUBJECTS [J].
DRI, P ;
SORANZO, MR ;
CRAMER, R ;
MENEGAZZI, R ;
MIOTTI, V ;
PATRIARCA, P .
INFLAMMATION, 1985, 9 (01) :21-31
[6]  
FUKUDA T, 1985, AM REV RESPIR DIS, V132, P981
[7]  
GARTNER I, 1980, IMMUNOLOGY, V40, P133
[8]   AN IMPROVED IMMUNOMAGNETIC PROCEDURE FOR THE ISOLATION OF HIGHLY PURIFIED HUMAN BLOOD EOSINOPHILS [J].
HANSEL, TT ;
DEVRIES, IJM ;
IFF, T ;
RIHS, S ;
WANDZILAK, M ;
BETZ, S ;
BLASER, K ;
WALKER, C .
JOURNAL OF IMMUNOLOGICAL METHODS, 1991, 145 (1-2) :105-110
[9]   HYPODENSE EOSINOPHILIC GRANULOCYTES IN NORMAL INDIVIDUALS AND PATIENTS WITH ASTHMA - GENERATION OF HYPODENSE CELL-POPULATIONS INVITRO [J].
KLOPROGGE, E ;
DELEEUW, AJ ;
DEMONCHY, JGR ;
KAUFFMAN, HF .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1989, 83 (02) :393-400
[10]   CELLULAR COMMUNICATION IN LEUKOTRIENE-C4 PRODUCTION BETWEEN EOSINOPHILS AND NEUTROPHILS [J].
KLOPROGGE, E ;
DELEEUW, AJ ;
DEMONCHY, JGR ;
KAUFFMAN, HF .
INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1989, 90 (01) :20-23