LOCALIZATION OF LOCUSTA-DP IN LOCUST CNS AND HEMOLYMPH SATISFIES INITIAL HORMONAL CRITERIA

被引：58

作者：

PATEL, M

CHUNG, JS

KAY, I

MALLET, AI

GIBBON, CR

THOMPSON, KSJ

BACON, JP

COAST, GM

机构：

[1] UNIV LONDON BIRKBECK COLL,DEPT BIOL,LONDON WC1E 7HX,ENGLAND

[2] ST THOMAS HOSP,ST JOHNS INST DERMATOL,LONDON SE1 7EH,ENGLAND

[3] UNIV SUSSEX,SCH BIOL SCI,SUSSEX CTR NEUROSCI,BRIGHTON BN1 9QG,E SUSSEX,ENGLAND

来源：

PEPTIDES | 1994年 / 15卷 / 04期

关键词：

DIURESIS; ELECTROSPRAY MASS SPECTROMETRY; IMMUNOCYTOCHEMISTRY; LOCUST;

D O I：

10.1016/0196-9781(94)90081-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Locusta-diuretic peptide (Locusta-DP) is a potent stimulant of fluid secretion and cyclic AMP production by locust Malpighian tubules. In this study, a polyclonal antiserum raised to the C-terminus of Locusta-DP reveals a wide distribution of immunoreactive cell bodies and processes throughout the CNS, and endings in two important neurohemal release sites: the corpora cardiaca and the perivisceral organs. HPLC fractionation of CNS, neurohemal structures, and hemolymph reveals immunoreactive material that coelutes with synthetic Locusta-DP and stimulates cyclic AMP production by locust tubules. The identity of the immunoreactive and biologically active material is confirmed as authentic Locusta-DP by mass spectrometry.

引用

页码：591 / 602

页数：12

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