RAPID PREPARATION OF SUBSARCOLEMMAL AND INTERFIBRILLAR MITOCHONDRIAL SUBPOPULATIONS FROM CARDIAC-MUSCLE

被引：16

作者：

CHEMNITIUS, JM

MANGLITZ, T

KLOEPPEL, M

DOENST, T

SCHWARTZ, P

KREUZER, H

ZECH, R

机构：

[1] UNIV GOTTINGEN,INST BIOCHEM,W-3400 GOTTINGEN,GERMANY

[2] UNIV GOTTINGEN,ZENTRUM ANAT,W-3400 GOTTINGEN,GERMANY

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1993年 / 25卷 / 04期

关键词：

D O I：

10.1016/0020-711X(93)90668-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. Subsarcolemmal and interfibrillar mitochondria were prepared with complete recovery from rabbit and porcine heart muscle by upward-flotation during 60 sec of Percoll(TM) density gradient centrifugation. 2. Mitochondrial subpopulations were identified and characterized according to buoyant density, electron-microscopy, marker enzyme activities and respiratory performance. 3. ADP-induced state 3-respiration related to latent citrate synthase activity as a marker for structurally intact mitochondria was not significantly different in both mitochondrial subtypes.

引用

页码：589 / 596

页数：8

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