HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 NEF INDUCES ACCUMULATION OF CD4 IN EARLY ENDOSOMES

被引：79

作者：

SCHWARTZ, O

DAUTRYVARSAT, A

GOUD, B

MARECHAL, V

SUBTIL, A

HEARD, JM

DANOS, O

机构：

[1] INST PASTEUR,UNITE BIOL INTERACT CELLULAIRES,F-75724 PARIS 15,FRANCE

[2] INST PASTEUR,UNITE GENET SOMAT,PARIS,FRANCE

来源：

JOURNAL OF VIROLOGY | 1995年 / 69卷 / 01期

关键词：

D O I：

10.1128/JVI.69.1.528-533.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have studied the fate of CD4 in CEM T cells expressing a human immunodeficiency virus type 1 H1V-1 Nef protein. Nef triggered a rapid endocytosis and a degradation of CD4, while most of the p56(lck) was upheld at the cell membrane. In the presence of Nef, CD 1 accumulated in acidic intracellular vesicles that were not stained by antibodies against rab6, a marker of the Golgi apparatus complex. Detection of transferrin in CD4 containing vesicles showed that CD4 was trapped in early endosomes, without significant accumulation of CD4 in late endocytic compartments. Internalization pathways taken by CD4 in Nef(+) cells may therefore be different from those observed after treatment with phorbol esters.

引用

页码：528 / 533

页数：6

共 32 条

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