GENOTYPING OF HEPATITIS-C VIRUS BY THE POLYMERASE CHAIN-REACTION - A COMPARISON OF 2 PCR-BASED METHODS USING CORE OR NS5 PRIMERS

被引：1

作者：

HASHIMOTO, M ^{[1
]}

CHAYAMA, K ^{[1
]}

TUBOTA, A ^{[1
]}

KOIDA, I ^{[1
]}

SAITOH, S ^{[1
]}

ARASE, Y ^{[1
]}

IKEDA, K ^{[1
]}

KOBAYASHI, M ^{[1
]}

KANDA, M ^{[1
]}

IWASAKI, S ^{[1
]}

NAKANO, A ^{[1
]}

TAKAGI, K ^{[1
]}

KOIKE, H ^{[1
]}

KUMADA, H ^{[1
]}

机构：

[1] OKINAKA MEM INST MED RES,MINATO KU,TOKYO 105,JAPAN

来源：

INTERNATIONAL HEPATOLOGY COMMUNICATIONS | 1995年 / 4卷 / 02期

关键词：

HCV RNA; CORE REGION; NS5; REGION;

D O I：

10.1016/0928-4346(95)00216-6

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

The presence of hepatitis C virus subtypes and their clinical importance, especially in the context of interferon sensitivity, are reported. In this study, we compared two kinds of polymerase chain reaction (PCR)-based methods - one step quick subtyping and two stage subtyping procedure for the determination of genotypes. Using the mixed primer sets derived from the core or NS5 region, we amplified cDNA samples obtained from patients with a hepatitis C virus infection. Of 116 samples tested, 91 (78%) showed identical genosubtypes between these two methods and 19 (16%) showed different ones, Although such discrepant results are considered to be related to the characteristics of these two methods, further studies remain to elucidated.

引用

页码：67 / 71

页数：5

共 4 条

[1]

Simmonds, McOmish, Yap, Et al., Sequence variability in the 5′ non-cording region of hepatitis C virus, J Gen Virol, 74, pp. 661-668, (1993)

[2]

Chayama, Tsubota, Arase, Et al., Genotypic subtyping of hepatitis C virus, J Gastroenterol Hepatol, 8, pp. 150-156, (1993)

[3]

Okamoto, Sugitama, Okada, Et al., Typing hepatitis C virus by polymerase chain reaction with type-specific primers: application to clinical surveys and tracing infections sources, J Gen Virol, 73, pp. 673-679, (1992)

[4]

Tsubota, Chayama, Ikeda, Et al., Factors Predictive of Response to Interferon-α Therapy in Hepatitis C virus Infection, Hepatology, 19, pp. 1088-1094, (1994)

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