C-TERMINAL AMIDATED PEPTIDES - PRODUCTION BY THE IN-VITRO ENZYMATIC AMIDATION OF GLYCINE-EXTENDED PEPTIDES AND THE IMPORTANCE OF THE AMIDE TO BIOACTIVITY

被引：198

作者：

MERKLER, DJ

机构：

[1] Analytical Protein and Organic Chemistry Group, Unigene Laboratories, Inc., Fairfield, NJ

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1994年 / 16卷 / 06期

关键词：

AMIDATED PEPTIDES; C-TERMINAL AMIDATION; PEPTIDYLGLYCINE ALPHA-AMIDATING ENZYME;

D O I：

10.1016/0141-0229(94)90014-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Peptidylglycine alpha-amidating enzyme (alpha-AE) can be used in an in vitro reaction to convert C-terminal glycine-extended peptides to peptide hormones with a C-terminal amino acid amide. Structure-activity data for 45 bioactive peptides show that the C-terminal amide is required for the full biological activity of most amidated peptide hormones. These data emphasize the role alpha-AE can have in amidated peptide production.

引用

页码：450 / 456

页数：7

共 92 条

[1] STRUCTURE-FUNCTION STUDIES ON NEUROHORMONE-D - ACTIVITY OF NATURALLY-OCCURRING HORMONE ANALOGS [J].