DIFFERENTIATION OF ISOLATES OF DISCULA-UMBRINELLA (TELEOMORPH, APIOGNOMONIA-ERRABUNDA) FROM BEECH, CHESTNUT, AND OAK USING RANDOMLY AMPLIFIED POLYMORPHIC DNA MARKERS

被引：49

作者：

HAEMMERLI, UA ^{[1
]}

BRANDLE, UE ^{[1
]}

PETRINI, O ^{[1
]}

MCDERMOTT, JM ^{[1
]}

机构：

[1] SWISS FED INST TECHNOL,INST MICROBIOL,CH-8092 ZURICH,SWITZERLAND

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 1992年 / 5卷 / 06期

关键词：

CASTANEA-SATIVA; FAGUS-SYLVATICA; QUERCUS SPP;

D O I：

10.1094/MPMI-5-479

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genetic variation in 30 isolates of Discula umbrinella derived from beech, chestnut, and oak was assessed using randomly amplified polymorphic DNA (RAPD) and restriction fragment length polymorphic markers. Polymerase chain reaction amplifications with 17 primers produced 134 different DNA fragments. Three RAPD fragments were subsequently used for Southern hybridization. By these techniques up to four different individuals could be detected in the same leaf. The presence of several individuals within a single leaf indicates a finely tuned balance between the endophyte and its host. Cluster analysis of all arbitrary primed amplified DNA fragments showed that the isolates could be placed into four groups corresponding to their host origin. The high percentage of private RAPD variants within groups is consistent with low gene flow.

引用

页码：479 / 483

页数：5