PURIFICATION OF MOUSE REN-2 PRORENIN PRODUCED IN CHINESE HAMSTER OVARY CELLS

被引：17

作者：

HATSUZAWA, K

KIM, WS

MURAKAMI, K

NAKAYAMA, K

机构：

[1] UNIV TSUKUBA,CTR GENE EXPT,SAKURA,IBARAKI 305,JAPAN

[2] UNIV TSUKUBA,INST APPL BIOCHEM,SAKURA,IBARAKI 305,JAPAN

[3] UNIV TSUKUBA,INST BIOL SCI,SAKURA,IBARAKI 305,JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1990年 / 107卷 / 06期

关键词：

D O I：

10.1093/oxfordjournals.jbchem.a123137

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Renin is produced from a larger, inactive precursor, prorenin, by endoproteolytic removal of the amino-terminal prosegment. In this study, we have transfected Chinese hamster ovary cells with the expression plasmid of mouse Ren 2 preprorenin, and have purified mouse Ren 2 prorenin from the incubation medium of these cells by DEAE-Toyopearl chromatography, Blue-Toyopearl chromatography, and isoelectric focusing. Prorenin thus purified has a molecular mass of 42 kDa as determined by SDS-PAGE and an isoelectric point of 6.5. Amino-terminal sequencing has demonstrated that the purified prorenin has the amino-terminus predicted from the nucleotide sequence of mouse Ren 2 preprorenin cDNA. © 1990 COPYRIGHT, 1990 BY THE JOURNAL OF BIOCHEMISTRY.

引用

页码：854 / 857

页数：4

共 26 条

[1] AN INACTIVE, PRORENIN-LIKE SUBSTANCE IN HUMAN-KIDNEY AND PLASMA [J].