CORTICOTROPIN RELEASING HORMONE MESSENGER-RNA IS ELEVATED ON THE AFTERNOON OF PROESTRUS IN THE PARVOCELLULAR PARAVENTRICULAR NUCLEI OF THE FEMALE RAT

被引:135
作者
BOHLER, HCL
ZOELLER, RT
KING, JC
RUBIN, BS
WEBER, R
MERRIAM, GR
机构
[1] UNIV MISSOURI,COLUMBIA SCH MED,DEPT ANAT,COLUMBIA,MO 65212
[2] TUFTS UNIV,SCH MED,DEPT ANAT & CELL BIOL,BOSTON,MA 02111
来源
MOLECULAR BRAIN RESEARCH | 1990年 / 8卷 / 03期
基金
美国国家卫生研究院; 美国国家科学基金会;
关键词
Corticotropin releasing hormone; Estrous cycle; In situ hybridization; Luteinizing hormone surge; mRNA; Paraventricular nucleus;
D O I
10.1016/0169-328X(90)90025-9
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We investigated changes during the estrous cycle in cellular levels of corticotropin-releasing hormone (CRH) mRNA in parvocellular neurons of the hypothalamic paraventricular nucleus, using in situ hybridization. Intact female rats with 4 day cycles were sacrificed at 11 different times during the cycle at 09.00 h and 16.00 h on each day, with additional collection times at 14.00 h, 18.00 h, and 20.00 h on the day of proestrus. Twelve μm coronal sections of fresh-frozen brains were made through the paraventricular nuclei (PVN) and placed on gelatin-coated slides. A 48 base oligodeoxynucleotide probe complementary to the coding region for rat CRH was used to measure CRH mRNA. There was a sharp increase (P < 0.01) in CRH mRNA in the ventral PVN between 14.00 and 16.00 h on the day of proestrus, at the approximate time of the ovulatory surge. Following this rise, there was an even larger decline (P < 0.01) between P 16.00 h and P 20.00 h. Levels of CRH mRNA did not change greatly on other days of the cycle, nor were there significant changes in the dorsal PVN. Given the known effects of CRH on GnRH secretion, these changes occur at a time when they could serve to modulate the midcycle luteinizing hormone (LH) surge. © 1990.
引用
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页码:259 / 262
页数:4
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