IDENTIFICATION AND PRELIMINARY CHARACTERIZATION OF WHITE STURGEON (ACIPENSER-TRANSMONTANUS) VITELLOGENIN MESSENGER-RNA

被引:19
作者
BIDWELL, CA
KROLL, KJ
SEVERUD, E
DOROSHOV, SI
CARLSON, DM
机构
[1] UNIV CALIF DAVIS,DEPT ANIM SCI,DAVIS,CA 95616
[2] UNIV CALIF DAVIS,DEPT BIOCHEM & BIOPHYS,DAVIS,CA 95616
关键词
D O I
10.1016/0016-6480(91)90147-X
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Vitellogenesis was induced in white sturgeon by administration of estrogen through silastic implants. Vitellogenin mRNA was identified by agarose gel electrophoresis and cell-free translation. A highly abundant 5.7-kb mRNA was induced in the liver of estrogentreated sturgeon. Cell-free translation of poly(A)+ mRNA showed the induction of two high-molecular-weight proteins of 180 and 120 kDa. These two proteins, encoded by the 5.7-kb mRNA(s), were immunoprecipitated by antiserum to serum from vitellogenic sturgeon. Immunoprecipitations also showed the presence of four other serum proteins synthesized by the liver of estrogen-treated sturgeon. The induction of vitellogenesis by estrogen in sturgeon, which are a primitive teleost, was found to be similar to induction of vitellogenesis in amphibians, avians, and other teleosts. Estrogen treatment induced a highly abundant vitellogenin mRNA as well as several mRNAs for other serum proteins. However, the presence of two distinct vitellogenin monomers in the cell-free translation assay was significantly different from the results in other species. © 1991.
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页码:415 / 424
页数:10
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