PRESERVATION OF PETUNIA POLLEN - METHODS INVITRO AND INVIVO VIABILITY TESTS AND GENETIC CONFORMITY

被引:3
作者
FARCY, E
VERHILLE, AM
CORNU, A
CERCEAULARRIVAL, MT
机构
来源
BULLETIN DE LA SOCIETE BOTANIQUE DE FRANCE-ACTUALITES BOTANIQUES | 1990年 / 137卷 / 02期
关键词
PETUNIA HYBRIDA; POLLEN; PRESERVATION; FREEZE-DRYING; VARIABILITY; GENETIC CONFORMITY;
D O I
10.1080/01811789.1990.10827006
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Petunia hybrida, gives the opportunity for testing - the viability of fresh and stored pollens by germination in vitro - the quality of these pollens by the quantity and the quality of seeds obtained after pollination - the genetic conformity of the next generation from stored pollens. Different pollen sets - pollen stored at + 4-degrees-C and - 36-degrees-C without previous treatments, - pollen lyophilised and stored under normal atmosphere or under vacuum at + 4-degrees-C and - 36-degrees-C, are tested after storages of 1, 6 and 12 months. All these processings allowed a good preservation of pollen for a short storage however low temperature (- 36-degrees-C) seemed necessary for a longer one. Germination rate in vitro decreased during storage, except for lyophilised sets kept at - 36-degrees-C, these conditions might be the best for storage longer than 12 months. Genetic potential of the male gametophyte does not seem to be altered as showned by: 1) the good transmission of pollen genetic markers, 2) the absence of effect on height and earliness of F1 plants, 3) the absence of detectable increase of mutation rate in the next generation.
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页码:105 / 110
页数:6
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