THE IMMEDIATE EARLY GENES OF HUMAN CYTOMEGALOVIRUS UP-REGULATE EXPRESSION OF THE INTERLEUKIN-2 AND INTERLEUKIN-2 RECEPTOR GENES

Human cytomegalovirus (HCMV) immediate early (IE) genes act as trans-acting factors to upregulate various viral promoters. We used various IE plasmid constructs in transient transfection assays and demonstrated that the HCMV IE2 gene product upregulated expression from the interleukin (IL)-2 and IL-2 receptor (IL-2R) promoters and increased amounts of endogenous, steady-state IL-2 and lL-2R RNA. In marked contrast, the IE1 gene product, which can upregulate the major IE promoter and the IL-1-beta promoter, had no effect on the IL-2 and lL-2R promoters. These studies suggest a role for the HCMV IE2 gene product as a modulator of the inflammatory response associated with HCMV infection.