EXPRESSION OF GABA-A RECEPTOR POLYPEPTIDES IN CLONAL RAT-CELL LINES

被引：9

作者：

KASCKOW, JW

TILLAKARATNE, NJK

KIM, H

STRECKER, GJ

TOBIN, AJ

OLSEN, RW

机构：

[1] UNIV CALIF LOS ANGELES,MENTAL RETARDAT RES CTR,LOS ANGELES,CA 90024

[2] UNIV CALIF LOS ANGELES,DEPT BIOL,LOS ANGELES,CA 90024

[3] UNIV CALIF LOS ANGELES,DEPT PSYCHIAT,LOS ANGELES,CA 90024

[4] UNIV CALIF LOS ANGELES,DEPT PHARMACOL,LOS ANGELES,CA 90024

[5] UNIV CALIF LOS ANGELES,BRAIN RES INST,LOS ANGELES,CA 90024

来源：

BRAIN RESEARCH | 1992年 / 581卷 / 01期

关键词：

GAMMA-AMINOBUTYRIC ACIDA RECEPTOR; CLONAL CELL; RECEPTOR SUBTYPE; BENZODIAZEPINE; NORTHERN BLOT; BINDING;

D O I：

10.1016/0006-8993(92)90353-B

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The neuronal-like cell lines, B35, B65, B103, and B104, previously reported to possess high affinity GABA binding, were analyzed for various cellular properties. They possessed peripheral but lacked central benzodiazepine binding. Only B65 cells possessed [H-3]muscimol binding; none bound [S-35]TBPS. None of the cells exhibited GABA-stimulated chloride conductance with patch clamp recordings. By Western blots the cells possessed alpha-subunits. Northern blot and polymerase chain reaction analysis showed that out of alpha-1, alpha-4, beta-1 and gamma-2 subunits, only alpha-1 subunit mRNA was present. Thus, GABA(A)-receptor binding without associated central benzodiazepine receptor sites and without functional chloride channels appears to result from expression of an incomplete subunit composition.

引用

页码：143 / 147

页数：5

共 30 条

[1] SINGLE SUBUNITS OF THE GABAA RECEPTOR FORM ION CHANNELS WITH PROPERTIES OF THE NATIVE RECEPTOR [J].