COMPARISON OF THE N-GLYCOLOYLNEURAMINIC AND N-ACETYLNEURAMINIC ACID CONTENT OF PLATELETS AND THEIR PRECURSORS USING HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY

被引：8

作者：

BUDD, TJ

DOLMAN, CD

LAWSON, AM

CHAI, W

SAXTON, J

HEMMING, FW

机构：

[1] QUEENS MED CTR, SCH MED, DEPT BIOCHEM, NOTTINGHAM NG7 2UH, ENGLAND

[2] MRC, CLIN RES CTR, CLIN MASS SPECTROMETRY SECT, HARROW HA1 3UJ, MIDDX, ENGLAND

来源：

GLYCOCONJUGATE JOURNAL | 1992年 / 9卷 / 05期

基金：

英国惠康基金;

关键词：

N-GLYCOLOYLNEURAMINIC ACID; N-ACETYLNEURAMINIC ACID; PROMEGAKARYOBLAST; PLATELET; HIGH PERFORMANCE ANION EXCHANGE CHROMATOGRAPHY;

D O I：

10.1007/BF00731139

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

N-Acetylneuraminic acid (Neu5Ac) and N-glycoloylneuraminic acid (Neu5Gc) are distributed widely in nature. Using a Carbopac PA-1 anion exchange column, we have determined the ratios of Neu5Ac and Neu5Gc in hydrolysates of platelets and their precursors: a rat promegakaryoblastic (RPM) cell line and a human megakaryoblastic leukemia cell line (MEG-01). The ratio of Neu5Gc:Neu5Ac in cultured RPM cells is 16:1, whereas in platelet rich plasma and cultured MEG-01 cells it is 1:38 and 1:28, respectively. The nature of these sialic acids from RPM cells was verified using thin layer chromatography and liquid secondary ion mass spectrometry. The relevance of increased Neu5Gc levels in early stages of development is discussed.

引用

页码：274 / 278

页数：5

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