FACTORS IMPROVING L-THREONINE PRODUCTION BY A 3 L-THREONINE BIOSYNTHETIC GENES AMPLIFIED RECOMBINANT STRAIN OF BREVIBACTERIUM-LACTOFERMENTUM

被引：15

作者：

ISHIDA, M

KAWASHIMA, H

SATO, K

HASHIGUCHI, K

ITO, H

ENEI, H

NAKAMORI, S

机构：

[1] Central Research Laboratories of Ajinomoto Co., Inc., Kanagawa, 1-1, Suzuki-cho, Kawasaki-ku

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1994年 / 58卷 / 04期

关键词：

D O I：

10.1271/bbb.58.768

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

When a Brevibacterium lactofermentum L-threonine producer that accumulated L-lysine as well, was transformed with a recombinant plasmid carrying the indigenous hom, thrB, and thrC genes, delayed growth and plasmid instability were observed. Addition of organic nutrients, vitamins (thiamine.HCl and d-biotin), and NaCl under the optimal culture conditions solved these problems and further increased threonine production in a small jar fermentor.

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页码：768 / 770

页数：3

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