INVITRO STUDIES OF CHEMICAL MUTAGENS AND CARCINOGENS .1. STABILITY STUDIES IN CELL-CULTURE MEDIUM

被引：42

作者：

JENSEN, EM ^{[1
]}

LAPOLLA, RJ ^{[1
]}

KIRBY, PE ^{[1
]}

HAWORTH, SR ^{[1
]}

机构：

[1] EG&G MASON RES INST, ROCKVILLE, MD 20852 USA

来源：

JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE | 1977年 / 59卷 / 03期

关键词：

D O I：

10.1093/jnci/59.3.941

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A quantitative Salmonella mutagenesis assay was used to study the stability of known mutagenic and carcinogenic compounds in cell culture medium. Ten direct acting carcinogens, when incubated in culture medium with 15% fetal bovine serum at pH 7.2-7.4 and 37.degree. C, became inactive at varying rates. Biologic half-lives of the test compounds ranged from 8 min to 67 h. Six procarcinogens showed no significant inactivation after 3 wk incubation. The biologic half-lives of each compound were presented, and the significance of these findings as they relate to cell culture carcinogenesis and mutagenesis assays was dicussed.

引用

页码：941 / 944

页数：4

共 18 条

[1] METHODS FOR DETECTING CARCINOGENS AND MUTAGENS WITH SALMONELLA-MAMMALIAN-MICROSOME MUTAGENICITY TEST [J].