CHROMATIN STRUCTURE OF DROSOPHILA-MELANOGASTER RIBOSOMAL GENES

被引：15

作者：

BELIKOV, SV

DZHERBASHYAJAN, AR

PREOBRAZHENSKAYA, OV

KARPOV, VL

MIRZABEKOV, AD

机构：

[1] W.A. Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow

来源：

FEBS LETTERS | 1990年 / 273卷 / 1-2期

关键词：

Chromatin structure; D; melanogaster; DNA-protein crosslinking; Ribosomal gene;

D O I：

10.1016/0014-5793(90)81085-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The chromatin structure of ribosomal genes of D. melanogaster has been studied by crosslinking proteins to DNA. We found that a number of histone contacts with DNA through histidine in the approximately 1 kb-long region surrounding the transcription initiation site, coding regions and the region of 240 bp-long repeats from the intergenic spacers (Alu-repeats) were weakened as compared to the inactive chromatin of the type II insertion. A protein with the molecular mass of 50 kDa (p50), associated with all DNA sequences analysed, has been discovered. Another protein with molecular mass of about 70 kDa (p70) has been found to be specific only for the Alu-repeats. © 1990.

引用

页码：205 / 207

页数：3

共 19 条

[1] SEQUENCE ORGANIZATION OF THE SPACER IN THE RIBOSOMAL GENES OF XENOPUS-CLIVII AND XENOPUS-BOREALIS
BACH, R
ALLET, B
CRIPPA, M
[J]. NUCLEIC ACIDS RESEARCH, 1981, 9 (20) : 5311 - 5330
[2] GENOMIC SEQUENCING
CHURCH, GM
GILBERT, W
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (07): : 1991 - 1995
[3] MULTIPLE POL-I INITIATION SEQUENCES IN RDNA SPACERS OF DROSOPHILA-MELANOGASTER
COEN, ES
DOVER, GA
[J]. NUCLEIC ACIDS RESEARCH, 1982, 10 (21) : 7017 - 7026
[4] EXPRESSION OF RIBOSOMAL INSERTION IN DROSOPHILA - SENSITIVITY TO INTERCALATING DRUGS
DAWID, IB
REBBERT, ML
[J]. NUCLEIC ACIDS RESEARCH, 1986, 14 (03) : 1267 - 1277
[5] A HIGHLY BASIC HISTONE-H4 DOMAIN BOUND TO THE SHARPLY BENT REGION OF NUCLEOSOMAL DNA
EBRALIDSE, KK
GRACHEV, SA
MIRZABEKOV, AD
[J]. NATURE, 1988, 331 (6154) : 365 - 367
[6] ESCHALIER G, 1970, IN VITRO, V6, P162
[7] INVIVO INTERACTIONS OF RNA POLYMERASE-II WITH GENES OF DROSOPHILA-MELANOGASTER
GILMOUR, DS
LIS, JT
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (08) : 2009 - 2018
[8] THE RARE TRANSCRIPTS OF INTERRUPTED RIBOSOMAL-RNA GENES IN DROSOPHILA-MELANOGASTER ARE PROCESSED OR DEGRADED DURING SYNTHESIS
JAMRICH, M
MILLER, OL
[J]. EMBO JOURNAL, 1984, 3 (07) : 1541 - 1545
[9] NON-TRANSCRIBED SPACER SEQUENCES PROMOTE INVITRO TRANSCRIPTION OF DROSOPHILA RIBOSOMAL DNA
KOHORN, BD
RAE, PMM
[J]. NUCLEIC ACIDS RESEARCH, 1982, 10 (21) : 6879 - 6886
[10] KOLCHINSKY AM, 1981, MOL BIOL USSR, V16, P302

← 1 2 →