MK-801 BLOCKS NICOTINIC DEPOLARIZATIONS OF GUINEA-PIG MYENTERIC NEURONS

被引：31

作者：

GALLIGAN, JJ ^{[1
]}

NORTH, RA ^{[1
]}

机构：

[1] OREGON HLTH SCI UNIV,VOLLUM INST,PORTLAND,OR 97201

来源：

NEUROSCIENCE LETTERS | 1990年 / 108卷 / 1-2期

关键词：

Hexamethonium; MK-801; Myenteric plexus; Nicotinic receptor; Serotonin-3; receptor;

D O I：

10.1016/0304-3940(90)90714-K

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Intracellular recordings were made from guinea-pig myenteric neurons. Acetylcholine (ACh), applied by iontophoresis, produced a depolarization that was blocked by hexamethonium (EC50 3.2 μM) but not by scopolamine (1 μM). MK-801 (0.3-30 μM), a non-competitive antagonist at N-methyl-d-aspartate receptors, also blocked the nicotinic depolarization (EC50 = 4.5 μM). Voltage clamp measurements of membrane current showed that MK-801 (1 μM) and hexamethonium (1 μM) both produced a greater inhibition of ACh-induced inward currents at -100 mV than at -40 mV. MK-801 (10 μM) did not change the depolarization evoked by 5-HT (acting at 5-HT3 receptors) in the same neurons in which it reduced the nicotinic response by more than 70%. It is concluded that MK-801 can act as a non-competitive antagonist of nicotinic responses in myenteric neurons. © 1990.

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页码：105 / 109

页数：5

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