REDUCTION OF RUBELLA ELISA BACKGROUND USING HEAT DENATURED SAMPLE BUFFER

被引：10

作者：

MAURACHER, CA

MITCHELL, LA

TINGLE, AJ

机构：

[1] UNIV BRITISH COLUMBIA,DEPT PEDIAT,VANCOUVER V5Z 4H4,BC,CANADA

[2] UNIV BRITISH COLUMBIA,DEPT PATHOL,VANCOUVER V5Z 4H4,BC,CANADA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1991年 / 145卷 / 1-2期

基金：

英国医学研究理事会;

关键词：

ELISA; RUBELLA; IMMUNOASSAY;

D O I：

10.1016/0022-1759(91)90334-C

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

During the analysis of rubella-specific antibodies in sera from adult vaccinees, it was observed that the inclusion of heat-denatured blocking proteins in the sample dilution buffer substantially reduced non-specific binding of serum IgG to microtitre plates. Results suggest that this modification to standard ELISA technique may reduce the incidence of false positive results in these assays.

引用

页码：251 / 254

页数：4

共 11 条

[1]

DORSETT PH, 1985, REV INFECT DIS, V7, pS150

[2] SOME MONOCLONAL-ANTIBODIES RAISED WITH A NATIVE PROTEIN BIND PREFERENTIALLY TO THE DENATURED ANTIGEN [J].