CLONING AND EXPRESSION IN ESCHERICHIA-COLI OF THE CDNA-ENCODING HUMAN CARDIAC TROPONIN-I

被引：10

作者：

ARMOUR, KL ^{[1
]}

HARRIS, WJ ^{[1
]}

TEMPEST, PR ^{[1
]}

机构：

[1] UNIV ABERDEEN MARISCHAL COLL,DEPT MOLEC & CELL BIOL,ABERDEEN AB9 1AS,SCOTLAND

来源：

GENE | 1993年 / 131卷 / 02期

关键词：

RECOMBINANT DNA; NUCLEOTIDE SEQUENCE; BETA-GALACTOSIDASE FUSION PROTEIN; T7 RNA POLYMERASE;

D O I：

10.1016/0378-1119(93)90308-P

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have cloned and sequenced the human cardiac troponin I (cTnI)-encoding cDNA with the aim of expressing the cDNA in Escherichia coli. The cDNA was successfully expressed as a fusion product with beta-galactosidase and as an unfused protein. Both polypeptides were recognised by an anti-human cTnI antibody.

引用

页码：287 / 292

页数：6

共 22 条

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