IN-VITRO ROOTING OF MICROPROPAGATED SHOOTS FROM JUVENILE AND MATURE PINUS-PINASTER EXPLANTS - INFLUENCE OF ACTIVATED-CHARCOAL

被引:66
作者
DUMAS, E [1 ]
MONTEUUIS, O [1 ]
机构
[1] ASSOC FORET CELLULOSE,BIOTECHNOL STN,F-77370 NANGIS,FRANCE
关键词
ADVENTITIOUS ROOTS; CONIFER; MICROCUTTINGS; ROOTING MEDIUM; TISSUE CULTURE;
D O I
10.1007/BF00048128
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The ability for adventitious rooting of micropropagated shoots from juvenile and mature Pinus pinaster Ait. explants was assessed in vitro on a rooting expression medium. The different rooting traits observed, namely the rooting rate, the number and the length of the adventitious roots, and the root score, were greatly influenced by the age of the donor plant: 98% of juvenile explants rooted, while only 49% of mature explants did. Addition of activated charcoal in the rooting expression medium improved the overall rooting capacity of the mature explants to an average of 78%. Whatever the plant material, the number and the length of the adventitious roots, as well as the root score, fluctuated according to the sampling date.
引用
收藏
页码:231 / 235
页数:5
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