SRFI, A NEW TYPE-II RESTRICTION ENDONUCLEASE THAT RECOGNIZES THE OCTANUCLEOTIDE SEQUENCE, 5'-GCCC/GGGC-3'/CGGG/CCCG

被引：11

作者：

SIMCOX, TG ^{[1
]}

MARSH, SJ ^{[1
]}

GROSS, EA ^{[1
]}

LERNHARDT, W ^{[1
]}

DAVIS, S ^{[1
]}

SIMCOX, MEC ^{[1
]}

机构：

[1] STRATAGENE CLONING SYST INC,11099 N TORREY PINES RD,LA JOLLA,CA 92037

来源：

GENE | 1991年 / 109卷 / 01期

关键词：

RECOMBINANT DNA; ENZYME IDENTIFICATION; NUCLEOTIDE SEQUENCE; STREPTOMYCES;

D O I：

10.1016/0378-1119(91)90596-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A new restriction endonuclease, SrfI has been isolated from an unidentified species of Streptomyces. SrfI recognizes the 8-bp palindrome, 5'-GCCCGGGC and cleaves double-stranded DNA after the third C in the sequence, producing blunt ends. SrfI is a rare-cutting enzyme and should therefore be useful for megabase mapping.

引用

页码：121 / 123

页数：3

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