AN ALTERNATIVE TO DNA EXTRACTION FOR THE DIAGNOSIS OF PNEUMOCYSTIS-CARINII PNEUMONIA BY POLYMERASE CHAIN-REACTION USING A NEW OLIGONUCLEOTIDE PROBE

被引：7

作者：

BORENSZTEIN, L

HATIN, I

SIMONPOLI, AM

UGARTE, E

GIRARD, PM

JAUREGUIBERRY, G

机构：

[1] HOP CLAUDE BERNARD,INSERM,U13,190 BLD MACDONALD,F-75994 PARIS 19,FRANCE

[2] HOP LOUIS MOURIER,SERV MED INTERNE,F-92700 COLOMBES,FRANCE

[3] GENSET,F-75011 PARIS,FRANCE

[4] HOP ROTHSCHILD,UNITE MALAD INFECT,F-75571 PARIS 12,FRANCE

来源：

MOLECULAR AND CELLULAR PROBES | 1992年 / 6卷 / 05期

关键词：

PNEUMOCYSTIS-CARINII; DNA AMPLIFICATION; OLIGONUCLEOTIDE PROBE;

D O I：

10.1016/0890-8508(92)90028-V

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used new specific primers and probe in a polymerase chain reaction (PCR) followed by Southern blot assays to detect Pneumocystis carinii in human bronchoalveolar lavage samples obtained from HIV-infected patients with pulmonary symptoms. To facilitate the procedure we developed a filtration technique without DNA extraction yielding a high sensitivity ( 18 18 positive results). The high specificity of the technique was shown by testing immunosuppressed patients without P. carinii pneumonia. © 1992.

引用

页码：361 / 365

页数：5

共 13 条

[1] RIBOSOMAL-RNA SEQUENCE SHOWS PNEUMOCYSTIS-CARINII TO BE A MEMBER OF THE FUNGI [J].