REQUIREMENT OF THE 2-HEADED STRUCTURE FOR THE PHOSPHORYLATION-DEPENDENT REGULATION OF SMOOTH-MUSCLE MYOSIN

被引：30

作者：

MATSUURA, M

IKEBE, M

机构：

[1] CASE WESTERN RESERVE UNIV,SCH MED,DEPT PHYSIOL & BIOPHYS,CLEVELAND,OH 44106

[2] MEIJI MILK PROD CO LTD,INST NUTR SCI,TOKYO 189,JAPAN

来源：

FEBS LETTERS | 1995年 / 363卷 / 03期

关键词：

MYOSIN; SMOOTH MUSCLE; PHOSPHORYLATION; MG2+-ATPASE ACTIVITY; BACULOVIRUS EXPRESSION;

D O I：

10.1016/0014-5793(95)00326-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It is known for smooth muscle myosin that while acto-HMM ATPase activity is regulated by phosphorylation, acto-S-l ATPase activity is not regulated. To clarify the heavy chain structure required for the regulation, smooth muscle myosin containing 7 different lengths of the S-2 portion were expressed in Sf9 insect cells using Baculovirus expression system. Myosin containing longer than 991 residues of heavy chain formed a stable two-headed structure while myosin with shorter than 944 residues of heavy chain formed a single-headed structure, indicating that the residues Gln(945)-Asp(991) are critical for the fomation of the two-headed structure. The actin activated ATPase activity of myosin mutants having a two-headed structure was activated by phosphorylation while that of myosin mutants that failed to form the two-headed structure was completely independent of phosphorylation. These results suggest that the two-headed structure is critical for the phosphorylation-dependent regulation.

引用

页码：246 / 250

页数：5

共 32 条

[1]

Hartshorne, Physiology of the Gastrointestinal Tract, 1, pp. 423-482, (1987)

[2]

Sellers, Adelstein, The Enzymes, 18, pp. 381-418, (1987)

[3]

Kamm, Stull, Annu. Rev. Physiol., 51, pp. 299-313, (1989)

[4]

Pearson, Jakes, John, Kendrick-Jones, Kemp, FEBS Lett., 168, pp. 108-112, (1984)

[5]

Ikebe, Hartshorne, J. Biol. Chem., 260, pp. 10027-10031, (1985)

[6]

Ikebe, Hartshorne, Elizinga, J. Biol. Chem., 261, pp. 36-39, (1986)

[7]

Kamisoyama, Araki, Ikebe, Biochemistry, 33, pp. 840-847, (1994)

[8]

Ikebe, Hartshorne, Elzinga, J. Biol. Chem., 262, pp. 9569-9573, (1987)

[9]

Begnur, Robinson, Appella, Sellers, J. Biol. Chem., 262, pp. 7613-7617, (1987)

[10]

Nishikawa, Sellers, Adelstein, Hidaka, J. Biol. Chem., 259, pp. 8808-8814, (1984)

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