ISOLATION AND CHARACTERIZATION OF THE RAT GLUTAMINE SYNTHETASE-ENCODING GENE

被引：58

作者：

VANDEZANDE, L

LABRUYERE, WT

ARNBERG, AC

WILSON, RH

VANDENBOGAERT, AJW

DAS, AT

VANOORSCHOT, DAJ

FRIJTERS, C

CHARLES, R

MOORMAN, AFM

LAMERS, WH

机构：

[1] UNIV AMSTERDAM,DEPT ANAT & EMBRYOL,MOLEC ANAT SECT,MEIBERGDREEF 15,1105 AZ AMSTERDAM,NETHERLANDS

[2] UNIV GLASGOW,DEPT GENET,GLASGOW G12 8QQ,SCOTLAND

[3] STATE UNIV GRONINGEN,DEPT BIOCHEM,9700 AB GRONINGEN,NETHERLANDS

来源：

GENE | 1990年 / 87卷 / 02期

关键词：

exon; intron; liver; mRNA sequence; phage cloning vectors; Recombinant DNA;

D O I：

10.1016/0378-1119(90)90306-C

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

From a rat genomic library in phage λCharon4A, a complete glutamine synthetase-encoding gene was isolated. The gene is 9.5-10 kb long, consists of seven exons, and codes for two mRNA species of 1375 nucleotides (nt) and 2787 nt, respectively. For both mRNAs, full-lenght cDNAs containing a short poly(A) tract were identified. The sequences of the entire mRNA and of the exon-intron transitions were determined. The smaller mRNA is identical to the 5′ 1375 nt of the long mRNA and contains the entire protein-coding region. The position of the transcription start point was mapped. Within the first 118 bp of promoter sequence, a (T)ATAA-box, a CCAAT-box and an SP1-binding site were identified. © 1990.

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页码：225 / 232

页数：8

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