IMMUNOCHEMICAL DETECTION BY SPECIFIC ANTIBODY TO THROMBIN OF PROTHROMBIN CONFORMATIONAL-CHANGES UPON ADSORPTION TO ARTIFICIAL SURFACES

被引：18

作者：

CHUANG, HYK ^{[1
]}

ANDRADE, JD ^{[1
]}

机构：

[1] UNIV UTAH,SCH ENGN,DEPT BIOENGN,SALT LAKE CITY,UT 84112

来源：

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH | 1985年 / 19卷 / 07期

关键词：

POLYVINYL CHLORIDE - Chemical Reactions - PROTEINS - Adsorption;

D O I：

10.1002/jbm.820190707

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Polyclonal antihuman alpha -thrombin antibodies produced in rabbits reacted minimally ( less than 0. 05%) in solution with human prothrombin. However, when prothrombin was adsorbed to artificial surfaces such as polyvinyl chloride (PVC), the cross-reactivity of surface-bound prothrombin with antibody IgG to thrombin ( greater than 95% purity) was shown to be significantly enhanced. On PVC, the molar ratios of antibody IgG to thrombin/prothrombin approached the same level as that of antibody IgG to thrombin/thrombin when thrombin was adsorbed to the same material. The results appear to suggest that prothrombin adsorption to PVC has resulted in some molecular conformational changes so that immunologically the adsorbed prothrombin resembles that of adsorbed thrombin on the same PVC surface.

引用

页码：813 / 825

页数：13

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