ALU-PRIMED POLYMERASE CHAIN-REACTION FOR REGIONAL ASSIGNMENT OF 110 YEAST ARTIFICIAL CHROMOSOME CLONES FROM THE HUMAN X-CHROMOSOME - IDENTIFICATION OF CLONES ASSOCIATED WITH A DISEASE LOCUS

被引：79

作者：

NELSON, DL ^{[1
]}

BALLABIO, A ^{[1
]}

VICTORIA, MF ^{[1
]}

PIERETTI, M ^{[1
]}

BIES, RD ^{[1
]}

GIBBS, RA ^{[1
]}

MALEY, JA ^{[1
]}

CHINAULT, AC ^{[1
]}

WEBSTER, TD ^{[1
]}

CASKEY, CT ^{[1
]}

机构：

[1] BAYLOR UNIV,HOWARD HUGHES MED INST,HOUSTON,TX 77030

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 14期

关键词：

DNA FINGERPRINTING; DNA SEQUENCING; LOWE SYNDROME; SOMATIC CELL HYBRIDS;

D O I：

10.1073/pnas.88.14.6157

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Over 400 yeast artificial chromosome (YAC) clones were isolated from the human X chromosome, and 110 of these were assigned to regions defined by chromosome translocation and deletion breakpoints. Polymerase chain reaction using Alu primers was applied to YAC clones in order to generate probes, to identify overlapping clones, and to derive "fingerprints" and sequence data directly from total yeast DNA. Several clones were identified in regions of medical interest. One set of three overlapping clones was found to cross a chromosomal translocation implicated in Lowe syndrome. The regional assignment of groups of YAC clones provides initiation points for further attempts to develop large cloned contiguous sequences, as well as material for investigation of regions involved in genetic diseases.

引用

页码：6157 / 6161

页数：5

共 32 条

[1] MOLECULAR CHARACTERIZATION OF HUMAN X/Y TRANSLOCATIONS SUGGESTS THEIR ETIOLOGY THROUGH ABERRANT EXCHANGE BETWEEN HOMOLOGOUS SEQUENCES ON XP AND YQ [J].