INTRACELLULAR CALCIUM CHELATORS AND OXIDANT-INDUCED RENAL PROXIMAL TUBULE CELL-DEATH

被引：20

作者：

SCHNELLMANN, RG

机构：

[1] Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, Georgia

来源：

JOURNAL OF BIOCHEMICAL TOXICOLOGY | 1991年 / 6卷 / 04期

关键词：

RENAL PROXIMAL TUBULES; CELL DEATH; CALCIUM CHELATORS; OXIDATIVE STRESS; CYTOSOLIC FREE CALCIUM;

D O I：

10.1002/jbt.2570060410

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

The effect of intracellular calcium chelators on rabbit renal proximal tubule (RPT) cell death induced by t-butyl hydroperoxide (TBHP) and H2O2 was examined. Preincubation of RPT suspensions with 50-mu-M QUIN 2/AM completely prevented TBHP (0.5 mM) and H2O2 (2 mM) induced cell death [i.e., release of lactate dehydrogenase (LDH)]. QUIN 2/AM, BAPTA/AM, EGTA/AM, and FURA 2/AM, at 5-mu-M, decreased LDH release (at 6 hr) from 41% to 4%, 21%, 26%, and 33%, and decreased lipid peroxidation (at 1 hr) from 1.0 to 0.1, 0.4, 0.6, and 0.8 nmol MDA/mg protein, respectively, after TBHP exposure. Since oxidant-induced lipid peroxidation and cell death are iron-dependent in this model, these results suggest that the intracellular calcium chelators inhibit cell death by chelating iron.

引用

页码：299 / 303

页数：5

共 16 条

[1] CALCIUM CHELATOR QUIN-2 PREVENTS HYDROGEN-PEROXIDE-INDUCED DNA BREAKAGE AND CYTO-TOXICITY [J].

CANTONI, O ;

SESTILI, P ;

CATTABENI, F ;

BELLOMO, G ;

POU, S ;

COHEN, M ;

CERUTTI, P .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 182 (02) :209-212

[2] THE ROLE OF CALCIUM-IONS IN TOXIC CELL INJURY [J].

FARBER, JL .

ENVIRONMENTAL HEALTH PERSPECTIVES, 1990, 84 :107-111

[3]

GORNALL AG, 1949, J BIOL CHEM, V177, P751

[4] ROLE OF LIPID-PEROXIDATION IN RENAL PROXIMAL TUBULE CELL-DEATH INDUCED BY HALOALKENE CYSTEINE CONJUGATES [J].

GROVES, CE ;

LOCK, EA ;

SCHNELLMANN, RG .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 1991, 107 (01) :54-62

[5]

GRYNKIEWICZ G, 1985, J BIOL CHEM, V260, P3440

[6] BLEBBING, FREE CA-2+ AND MITOCHONDRIAL-MEMBRANE POTENTIAL PRECEDING CELL-DEATH IN HEPATOCYTES [J].

LEMASTERS, JJ ;

DIGUISEPPI, J ;

NIEMINEN, AL ;

HERMAN, B .

NATURE, 1987, 325 (6099) :78-81

[7] CALCIUM-ACTIVATED DNA FRAGMENTATION KILLS IMMATURE THYMOCYTES [J].

MCCONKEY, DJ ;

HARTZELL, P ;

NICOTERA, P ;

ORRENIUS, S .

FASEB JOURNAL, 1989, 3 (07) :1843-1849

[8]

NICOTERA P, 1986, J BIOL CHEM, V261, P4628

[9] CORRELATION BETWEEN CYTOSOLIC CA-2+ CONCENTRATION AND CYTO-TOXICITY IN HEPATOCYTES EXPOSED TO OXIDATIVE STRESS [J].

NICOTERA, P ;

MCCONKEY, D ;

SVENSSON, SA ;

BELLOMO, G ;

ORRENIUS, S .

TOXICOLOGY, 1988, 52 (1-2) :55-63

[10] CYTOSOLIC-FREE CA-2+ AND CELL KILLING IN HEPATOMA 1C1C7 CELLS EXPOSED TO CHEMICAL ANOXIA [J].

NICOTERA, P ;

THOR, H ;

ORRENIUS, S .

FASEB JOURNAL, 1989, 3 (01) :59-64

← 1 2 →