RNASE-H ACTIVITY OF HIV REVERSE TRANSCRIPTASES IS CONFINED EXCLUSIVELY TO THE DIMERIC FORMS

被引：63

作者：

RESTLE, T

MULLER, B

GOODY, RS

机构：

[1] Abteilung Biophysik, Max-Planck-Institut für medizinische Forschung, 9600 Heidelberg

来源：

FEBS LETTERS | 1992年 / 300卷 / 01期

关键词：

HIV; REVERSE TRANSCRIPTASE; RNASE-H; DIMERIZATION;

D O I：

10.1016/0014-5793(92)80172-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the rapid preparation of a defined substrate to monitor RNase H activity has been developed. Using this substrate, we have investigated the RNase H activities of the different forms of recombinant HIV-1 and HIV-2 reverse transcriptase (RT) in detail. As we report here, RNase H activity is associated only with the dimeric forms (p51/p66 or p66/p66) of the enzymes

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页码：97 / 100

页数：4

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