ISOZYME SPECIFIC POLYMERASE CHAIN-REACTION ANALYSIS OF DIFFERENTIAL GENE-EXPRESSION - A GENERAL-METHOD APPLIED TO LIGNIN PEROXIDASE GENES OF PHANEROCHAETE-CHRYSOSPORIUM

被引：13

作者：

BROOKS, P ^{[1
]}

SIMS, P ^{[1
]}

BRODA, P ^{[1
]}

机构：

[1] UNIV MANCHESTER,INST SCI & TECHNOL,DEPT BIOCHEM & APPL MOLEC BIOL,MANCHESTER M60 1QD,LANCS,ENGLAND

来源：

BIO-TECHNOLOGY | 1993年 / 11卷 / 07期

关键词：

D O I：

10.1038/nbt0793-830

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Analysis of differential expression of closely related genes is a general problem. The lignin peroxidase genes of Phanerochaete chrysosporium represent a typical case. They are differentially expressed according to the conditions encountered during growth. We show that the expression of two such genes, LIG1 and LIG5, can be differentiated at the mRNA level using a highly specific form of the polymerase chain reaction. This generally applicable method allows rapid and accurate analysis of complex and closely related mRNA populations.

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页码：830 / 834

页数：5

相关论文

共 25 条

[1] ABSOLUTE MESSENGER-RNA QUANTIFICATION USING THE POLYMERASE CHAIN-REACTION (PCR) - A NOVEL-APPROACH BY A PCR AIDED TRANSCRIPT TITRATION ASSAY (PATTY) [J].

BECKERANDRE, M ;

HAHLBROCK, K .

NUCLEIC ACIDS RESEARCH, 1989, 17 (22) :9437-9446

[2] MULTIPLE LIGNINASE-RELATED GENES FROM PHANEROCHAETE-CHRYSOSPORIUM [J].

BROWN, A ;

SIMS, PFG ;

RAEDER, U ;

BRODA, P .

GENE, 1988, 73 (01) :77-85

[3] EFFECT OF ENVIRONMENTAL-CONDITIONS ON EXTRACELLULAR PROTEASE ACTIVITY IN LIGNOLYTIC CULTURES OF PHANEROCHAETE-CHRYSOSPORIUM [J].

DOSORETZ, CG ;

CHEN, HC ;

GRETHLEIN, HE .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (02) :395-400

[4] ANALYSIS OF CYTOKINE MESSENGER-RNA AND DNA - DETECTION AND QUANTITATION BY COMPETITIVE POLYMERASE CHAIN-REACTION [J].

GILLILAND, G ;

PERRIN, S ;

BLANCHARD, K ;

BUNN, HF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (07) :2725-2729

[5] STRUCTURE AND REGULATION OF A LIGNIN PEROXIDASE GENE FROM PHANEROCHAETE-CHRYSOSPORIUM [J].

HOLZBAUR, ELF ;

MING, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 155 (02) :626-633

[6] EXPRESSION OF A SINGLE LIGNIN PEROXIDASE-ENCODING GENE IN PHANEROCHAETE-CHRYSOSPORIUM STRAIN ME446 [J].

JAMES, CM ;

FELIPE, MSS ;

SIMS, PFG ;

BRODA, P .

GENE, 1992, 114 (02) :217-222

[7] ENZYMATIC COMBUSTION - THE MICROBIAL-DEGRADATION OF LIGNIN [J].

KIRK, TK ;

FARRELL, RL .

ANNUAL REVIEW OF MICROBIOLOGY, 1987, 41 :465-505

[8] PRODUCTION OF MULTIPLE LIGNINASES BY PHANEROCHAETE-CHRYSOSPORIUM - EFFECT OF SELECTED GROWTH-CONDITIONS AND USE OF A MUTANT STRAIN [J].

KIRK, TK ;

CROAN, S ;

TIEN, M ;

MURTAGH, KE ;

FARRELL, RL .

ENZYME AND MICROBIAL TECHNOLOGY, 1986, 8 (01) :27-32

[9]

LEISOLA MSA, 1987, J BIOL CHEM, V262, P419

[10] PHENOTYPIC CLASSES OF PHENOLOXIDASE-NEGATIVE MUTANTS OF THE LIGNIN-DEGRADING FUNGUS PHANEROCHAETE-CHRYSOSPORIUM [J].

LIWICKI, R ;

PATERSON, A ;

MACDONALD, MJ ;

BRODA, P .

JOURNAL OF BACTERIOLOGY, 1985, 162 (02) :641-644