QUANTITATION OF THE GLYCATION INTERMEDIATE 3-DEOXYGLUCOSONE BY OXIDATION WITH RABBIT LIVER OXOALDEHYDE DEHYDROGENASE TO 2-KETO-3-DEOXYGLUCONIC ACID FOLLOWED BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：11

作者：

FUJII, E

IWASE, H

ISHIIKARAKASA, I

YAJIMA, Y

HOTTA, K

机构：

[1] KITASATO UNIV,SCH MED,DEPT INTERNAL MED,SAGAMIHARA,KANAGAWA 228,JAPAN

[2] KITASATO UNIV,SCH MED,DEPT BIOCHEM,SAGAMIHARA,KANAGAWA 228,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 660卷 / 02期

关键词：

D O I：

10.1016/0378-4347(94)00313-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and sensitive method for the detection of 3-deoxyglucosone was developed using oxidation with crude oxoaldehyde dehydrogenase to 2-keto-3-deoxygluconic acid followed by high-performance liquid chromatography (HPLC). Oxoaldehyde dehydrogenase was prepared from rabbit liver and partially characterized. 2-Keto-3-deoxygluconic acid produced from 3-deoxyglucosone by oxoaldehyde dehydrogenase was derivatized with 1,2-diamino-4,5-methylenedioxybenzene , and the fluorescent products were detected and quantitated by HPLC using a solvent containing berate. In the presence of berate, 2-keto-3-deoxygluconic acid was completely separated from N-acetylneuraminic acid. The detection limit of 3-deoxyglucosone was 2.5 pmol/injection (10 mu l) at a signal-to-noise ratio of 3. This method was used to confirm the inhibitory effect of aminoguanidine on glycation.

引用

页码：265 / 270

页数：6

共 21 条

[1] AMINOGUANIDINE PREVENTS DIABETES-INDUCED ARTERIAL-WALL PROTEIN CROSS-LINKING [J].