A 67-KDA PROTEIN-BINDING TO THE CURVED DNA IN THE MAIN REGULATORY REGION OF THE RAT MITOCHONDRIAL GENOME

被引：6

作者：

GADALETA, G ^{[1
]}

DELIA, D ^{[1
]}

SACCONE, C ^{[1
]}

PEPE, G ^{[1
]}

机构：

[1] UNIV BARI,CNR,CSMME,I-70126 BARI,ITALY

来源：

GENE | 1995年 / 160卷 / 02期

关键词：

PROTEIN-DNA COMPLEX; DNA REPLICATION; REGULATORY FACTOR; MITOPLAST; AFFINITY CHROMATOGRAPHY; D-LOOP;

D O I：

10.1016/0378-1119(95)00122-M

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have purified, by sequence-specific affinity chromatography, a mitochondrial (mt) matrix protein which binds to the curved DNA located between the replication origin (ori) of the leading strand (ori-H) and the two transcription promoters in the rat mt genome. The protein was characterized by gel electrophoresis as a 67-kDa polypeptide and seems to be involved in the DNA contact on the mt light strand. This protein differs (in the size and location of its DNA-binding site) from other DNA-binding proteins studied so far in animal mt systems. We suggest a role for the 67-kDa protein, assisted by other proteins, in regulating the initiation of leading-strand replication.

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页码：229 / 234

页数：6

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