DETERMINATION OF LY217332, A NEW 3'-QUATERNARY AMMONIUM CEPHALOSPORIN, IN PLASMA BY SOLID-PHASE COLUMN EXTRACTION AND HPLC

A sensitive and rapid assay has been developed for the determination of LY217332, a 3 prime -imidazolo left bracket 4, 5-c right bracket pyridinium cephalosporin, in plasma. The method utilizes cyano solid phase column extraction and HPLC with ultraviolet detection. The lower limit of detection is 5 ng/ml plasma and the relative standard deviation for precision and accuracy was 5% or less from 50-500 ng/ml. The method is applicable to the assay of ceftazidime, cephaloridine, cefpirome and BMY-28142 with minor modification of the mobile phase and the detection wavelength.