STUDIES OF RIBOSOMES OF YEAST SPECIES - SUSCEPTIBILITY TO INHIBITORS OF PROTEIN-SYNTHESIS INVIVO AND INVITRO

The susceptibility of cells from various species of Saccharomyces [S. cerevisiae, S. bisporus, S. douglasii, S. italicus, S. uvarum, S. bayanus, S. bailii, S. rouxii, S. delbrueckii, S. rosei, S. dairensis, S. kluyveri] and Kluyveromyces [K. fragils, K. dobzhanskii, K. marxianus, K. lactis, K. wickerhami] was tested against different types of protein synthesis inhibitors. Minimal inhibitory concentrations were determined for each yeast species and the sensitivity of their ribosomes in cell-free extracts was tested. Two aminoglycosides, paromomycin and hygromycin B, were assayed for capacity to stimulate translation errors with ribosomes of yeast species showing different minimal inhibitory concentrations in vivo. In many cases a correlation exists between natural and in vitro resistance, suggesting that some natural antibiotic resistances are ribosomal. [Yarrowia lipolytica was also studied.].