WILD-TYPE P53 BINDS TO THE TATA-BINDING PROTEIN AND REPRESSES TRANSCRIPTION

被引：564

作者：

SETO, E

USHEVA, A

ZAMBETTI, GP

MOMAND, J

HORIKOSHI, N

WEINMANN, R

LEVINE, AJ

SHENK, T

机构：

[1] PRINCETON UNIV,HOWARD HUGHES MED INST,PRINCETON,NJ 08544

[2] PRINCETON UNIV,DEPT MOLEC BIOL,PRINCETON,NJ 08544

[3] WISTAR INST,PHILADELPHIA,PA 19104

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 24期

关键词：

D O I：

10.1073/pnas.89.24.12028

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.

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页码：12028 / 12032

页数：5

相关论文

共 39 条

[1] OVERLAP OF THE P53-RESPONSIVE ELEMENT AND CAMP-RESPONSIVE ELEMENT IN THE ENHANCER OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I [J].

AOYAMA, N ;

NAGASE, T ;

SAWAZAKI, T ;

MIZUGUCHI, G ;

NAKAGOSHI, H ;

FUJISAWA, JI ;

YOSHIDA, M ;

ISHII, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (12) :5403-5407

[2] WILD-TYPE BUT NOT MUTANT P53 IMMUNOPURIFIED PROTEINS BIND TO SEQUENCES ADJACENT TO THE SV40 ORIGIN OF REPLICATION [J].

BARGONETTI, J ;

FRIEDMAN, PN ;

KERN, SE ;

VOGELSTEIN, B ;

PRIVES, C .

CELL, 1991, 65 (06) :1083-1091

[3] MOLECULAR ANALYSIS AND CHROMOSOMAL MAPPING OF AMPLIFIED GENES ISOLATED FROM A TRANSFORMED MOUSE 3T3-CELL LINE [J].

CAHILLYSNYDER, L ;

YANGFENG, T ;

FRANCKE, U ;

GEORGE, DL .

SOMATIC CELL AND MOLECULAR GENETICS, 1987, 13 (03) :235-244

[4] MODULATION OF ACTIVITY OF THE PROMOTER OF THE HUMAN MDR1 GENE BY RAS AND P53 [J].

CHIN, KV ;

UEDA, K ;

PASTAN, I ;

GOTTESMAN, MM .

SCIENCE, 1992, 255 (5043) :459-462

[5] ACCURATE TRANSCRIPTION INITIATION BY RNA POLYMERASE-II IN A SOLUBLE EXTRACT FROM ISOLATED MAMMALIAN NUCLEI [J].

DIGNAM, JD ;

LEBOVITZ, RM ;

ROEDER, RG .

NUCLEIC ACIDS RESEARCH, 1983, 11 (05) :1475-1489

[6] TUMORIGENIC POTENTIAL ASSOCIATED WITH ENHANCED EXPRESSION OF A GENE THAT IS AMPLIFIED IN A MOUSE-TUMOR CELL-LINE [J].

FAKHARZADEH, SS ;

TRUSKO, SP ;

GEORGE, DL .

EMBO JOURNAL, 1991, 10 (06) :1565-1569

[7] WILD-TYPE P53 ACTIVATES TRANSCRIPTION INVITRO [J].

FARMER, G ;

BARGONETTI, J ;

ZHU, H ;

FRIEDMAN, P ;

PRYWES, R ;

PRIVES, C .

NATURE, 1992, 358 (6381) :83-86

[8] PRESENCE OF A POTENT TRANSCRIPTION ACTIVATING SEQUENCE IN THE P53 PROTEIN [J].

FIELDS, S ;

JANG, SK .

SCIENCE, 1990, 249 (4972) :1046-1049

[9] ACTIVATING MUTATIONS FOR TRANSFORMATION BY P53 PRODUCE A GENE-PRODUCT THAT FORMS AN HSC70-P53 COMPLEX WITH AN ALTERED HALF-LIFE [J].

FINLAY, CA ;

HINDS, PW ;

TAN, TH ;

ELIYAHU, D ;

OREN, M ;

LEVINE, AJ .

MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (02) :531-539

[10] NEGATIVE EFFECT OF THE TRANSCRIPTIONAL ACTIVATOR GAL4 [J].

GILL, G ;

PTASHNE, M .

NATURE, 1988, 334 (6184) :721-724

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