ISOLATION OF DNA-SEQUENCES ON HUMAN CHROMOSOME-21 BY APPLICATION OF A RECOMBINATION-BASED ASSAY TO DNA FROM FLOW-SORTED CHROMOSOMES

被引：26

作者：

TANTRAVAHI, U

STEWART, GD

VANKEUREN, M

MCNEIL, G

ROY, S

PATTERSON, D

DRABKIN, H

LALANDE, M

KURNIT, DM

LATT, SA

机构：

[1] CHILDRENS HOSP MED CTR, DIV GENET, 300 LONGWOOD AVE, BOSTON, MA 02115 USA

[2] CHILDRENS HOSP MED CTR, CTR MENTAL RETARDAT, BOSTON, MA 02115 USA

[3] CHILDRENS HOSP MED CTR, HOWARD HUGHES MED INST, BOSTON, MA 02115 USA

[4] HARVARD UNIV, SCH MED, DEPT PEDIAT, BOSTON, MA 02115 USA

[5] HARVARD UNIV, SCH MED, DEPT GENET, BOSTON, MA 02115 USA

[6] UNIV COLORADO, HLTH SCI CTR, ELEANOR ROOSEVELT INST CANC RES, DENVER, CO 80206 USA

[7] UNIV MICHIGAN, MED CTR, HOWARD HUGHES MED INST, ANN ARBOR, MI 48109 USA

来源：

HUMAN GENETICS | 1988年 / 79卷 / 03期

关键词：

D O I：

10.1007/BF00366237

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

By merging two efficient technologies, bivariate flow sorting of human metaphase chromosomes and a recombination-based assay for sequence comlexity, we isolated 28 cloned DNA segments homologous to loci on human chromosome 21. Subregional mapping of these DNA segments with a somatic cell hybrid panel showed that 26 of the 28 cloned DNA sequences are distributed along the long arm of chromosome 21, while the other 2 hybridize with sequences on the short arm of both chromosome 21 and other chromosomes. This new collection of probes homologous to chromosome 21 should facilitate molecular analyses of trisomy 21 by providing DNA probes for the linkage map of chromosome 21, for studies of nondisjunction, for chromosome walking in clinically relevant subregions of chromosome 21, and for the isolation of genes on chromosome 21 following the screening of cDNA libraries.

引用

页码：196 / 202

页数：7

共 36 条

[1]

BRADLEY CM, 1986, TRISOMY, V21, P41

[2] ANALYSIS OF GENE-CONTROL SIGNALS BY DNA-FUSION AND CLONING IN ESCHERICHIA-COLI [J].