ISOLATION AND BIOCHEMICAL-CHARACTERIZATION OF A ZONA PELLUCIDA-BINDING GLYCOPROTEIN OF BOAR SPERMATOZOA

被引：61

作者：

JONAKOVA, V

SANZ, L

CALVETE, JJ

HENSCHEN, A

CECHOVA, D

TOPFERPETERSEN, E

机构：

[1] UNIV MUNICH, DEPT DERMATOL, ANDROL UNIT, FRAUENLOBSTR 9-11, W-8000 MUNICH 2, GERMANY

[2] INST MOLEC GENET, PRAGUE, CZECHOSLOVAKIA

[3] CSIC, INST QUIM PHYS, MADRID 6, SPAIN

[4] MAX PLANCK INST BIOCHEM, W-8033 MARTINSRIED, GERMANY

[5] UNIV CALIF IRVINE, INST MOLEC BIOL & BIOCHEM, IRVINE, CA 92717 USA

来源：

FEBS LETTERS | 1991年 / 280卷 / 01期

关键词：

BOAR SPERM; SPERM PROTEIN; ZONA PELLUCIDA-BINDING PROTEINS;

D O I：

10.1016/0014-5793(91)80233-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lectin-like molecules on the sperm surface are implicated in the process of gamete recognition and adhesion. We have isolated and biochemically characterized a 15 kDa glycoprotein from ejaculated boar sperm which possess zona pellucida-binding- and haemagglutinating-activity. The zona/15 kDa protein interaction is inhibited by fucoidan, suggesting that the sperm components which participate in the initial gamete interaction. N-Terminal sequence analysis of the isolated 15 kDa glycoprotein showed that it may belong to the same sperm/egg recognition-mediating protein family as the sea urchin sperm protein binding.

引用

页码：183 / 186

页数：4

共 33 条

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