2-DEOXY-D-GLUCOSE INHIBITS REJOINING OF RADIATION-INDUCED DNA DOUBLE-STRAND BREAKS IN YEAST

被引：15

作者：

FRANKENBERGSCHWAGER, M

HARBICH, R

FRANKENBERG, D

JAIN, V

机构：

[1] INST NUCL MED & ALLIED SCI,LUCKNOW MARG,DELHI 110054,INDIA

[2] GESELL STRAHLEN & UMWELTFORSCH MBH,INST BIOPHYS STRAHLENFORSCH,W-6000 FRANKFURT 70,GERMANY

来源：

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY | 1992年 / 61卷 / 02期

关键词：

D O I：

10.1080/09553009214550801

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Effects of 2-deoxy-D-glucose (2-DG) on radiation-induced DNA double-strand breaks (dsb) have been studied under non-growth conditions in a respiratory-deficient strain of the yeast Saccharomyces cerevisiae. Velocity sedimentation in neutral sucrose gradients was used to measure DNA dsb. Addition of 2-DG to the liquid-holding medium (67 mM phosphate buffer, pH 5, 30-degrees-C) at an equimolar concentration with glucose (50 mM) reduced the rate and extent of dsb rejoining. The inhibition of rejoining mediated by 2-DG is reversible for the majority - but not all - of the radiation-induced dsb.

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页码：185 / 190

页数：6

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