CONFORMATION OF HUMAN PLASMA-PROTEINS AT POLYMER SURFACES - THE EFFECTIVENESS OF SURFACE HEPARINIZATION

被引：60

作者：

BARBUCCI, R ^{[1
]}

MAGNANI, A ^{[1
]}

机构：

[1] UNIV SIENA,DIPARTIMENTO CHIM,I-53100 SIENA,ITALY

来源：

BIOMATERIALS | 1994年 / 15卷 / 12期

关键词：

POLYMER-PROTEIN INTERACTIONS; POLYURETHANES; HEPARINIZED SURFACES;

D O I：

10.1016/0142-9612(94)90075-2

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Studies were made on the adsorption of two human plasma proteins, albumin (HSA) and fibrinogen (HFg), onto three different polymeric surfaces: commercial pellethane(k) 2363-80AE (PU); pellethane crosslinked with a poly(amido-amine) (PUPA); and heparinized PUPA, using in situ ATR/FTIR spectroscopy (attenuated total reflection Fourier transform infrared spectroscopy). Conformational changes were found to occur on the two proteins upon adsorption onto bare PU and PUPA, and the protein unfolding on bare PU was also found to be time dependent. On the contrary, the two proteins do not change conformation when they are adsorbed onto the heparinized surface, emphasising the effectiveness of surface heparinization.

引用

页码：955 / 962

页数：8

共 30 条

[1] IN-VITRO BIOCOMPATIBILITY EVALUATION OF A HEPARINIZABLE MATERIAL (PUPA), BASED ON POLYURETHANE AND POLY(AMIDO-AMINE) COMPONENTS [J].