METABOLIC BURDEN AS REFLECTED BY MAINTENANCE COEFFICIENT OF RECOMBINANT ESCHERICHIA-COLI OVEREXPRESSING TARGET GENE

被引：58

作者：

BHATTACHARYA, SK ^{[1
]}

DUBEY, AK ^{[1
]}

机构：

[1] INDIAN INST TECHNOL,DEPT BIOCHEM ENGN & BIOTECHNOL,NEW DELHI 110016,INDIA

来源：

BIOTECHNOLOGY LETTERS | 1995年 / 17卷 / 11期

关键词：

D O I：

10.1007/BF00128377

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Metabolic burden as a consequence of overexpression of target gene, in a recombinant strain of E. coli 1727 has been analyzed with respect to maintenance energy coefficient (m). The values of 'm' for the host, uninduced recombinant and IPTG induced recombinant were determined to be 0.12, 0.17 and 0.32 g . g(-1). h(-1) respectively. Transient plasmid instability and nearly 33% fall in maximum specific growth rate were observed under conditions bf enhanced requirements for maintenance energy.

引用

页码：1155 / 1160

页数：6

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