NO EFFECT OF FACTOR-XA OR TISSUE THROMBOPLASTIN AND FACTOR-VII ON ACTIVATION OF PLASMINOGEN

A fast and simple method for quantitative determination of the Glu-plasminogen/Lys-plasminogen ratio was developed, in order to study a possible transformation between the plasminogen forms. Plasminogen [human] was incubated with factor Xa with and without cephalin and Ca2+ and with factor VII in presence of tissue thromboplastin. Samples were taken at different times. Urokinase was added to a final concentration of 1000 Ploug units/ml and the samples were incubated further for 15 min at 25.degree. C. The plasmin activity was determined and compared with a standard curve obtained by a similar activation of plasminogen samples with known Glu-plasminogen/Lys-plasminogen ratios. No effect was demonstrated by any of the coagulation factors tested.